Formulasi dan Uji Antibakteri Sediaan Sabun Padat Kombinasi Ekstrak Etanol Daun Pepaya Jepang (Cnidoscolus Aconitifolius) dan Minyak Serai Wangi (Cymbopogon Nardus L.) terhadap Bakteri Staphylococcus Aureus ATCC 25923
DOI:
https://doi.org/10.71417/galen.v2i2.298Kata Kunci:
Antibakteri, Cnidoscolus aconitifolius, Cymbopogon nardus, Sabun padat, Staphylococcus aureusAbstrak
Latar Belakang: Staphylococcus aureus merupakan bakteri penyebab infeksi kulit yang umum dijumpai. Bahan alam seperti minyak atsiri serai wangi (Cymbopogon nardus L.) dan ekstrak etanol daun pepaya Jepang (Cnidoscolus aconitifolius) berpotensi dikembangkan sebagai antibakteri dalam sediaan sabun padat. Tujuan: Penelitian ini bertujuan memformulasikan kombinasi ekstrak etanol daun pepaya Jepang dan minyak atsiri serai wangi dalam sediaan sabun padat, mengevaluasi mutu fisik, serta mengetahui aktivitas antibakterinya terhadap S. aureus ATCC 25923. Metode: Penelitian eksperimental laboratorium dilakukan dengan ekstraksi daun pepaya Jepang menggunakan metode maserasi etanol 70% dan perolehan minyak atsiri serai wangi melalui destilasi uap. Kombinasi bahan aktif diformulasikan menjadi tiga formula (F1, F2, dan F3). Evaluasi meliputi organoleptik, homogenitas, pH, tinggi busa, kadar air, asam lemak bebas, bahan tak larut etanol, lemak total, iritasi, dan hedonik. Aktivitas antibakteri diuji menggunakan metode difusi cakram. Hasil: Seluruh formula memenuhi persyaratan mutu fisik berdasarkan SNI dan menunjukkan aktivitas antibakteri. Formula dengan konsentrasi bahan aktif tertinggi menghasilkan daya hambat terbesar. Kesimpulan: Kombinasi kedua bahan aktif berhasil diformulasikan menjadi sabun padat yang memenuhi persyaratan mutu fisik dan memiliki aktivitas antibakteri terhadap S. aureus ATCC 25923.
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Referensi
Afrizal, Defitriani, A., & Efdi, M. (2024). Minyak atsiri serai wangi (Cymbopogon nardus L. Rendle): Diisolasi dengan dua metode berbeda, kualitas dan aktivitas antibakterinya. Jurnal Riset Kimia, 15(1), 99–111. https://doi.org/10.25077/jrk.v15i1.653
Anggraini, A. D., Wikantyasning, E. R., Farmasi, F., Muhammadiyah, U., & Tengah, J. (2025). Optimasi formulasi sediaan nanoemulgel ekstrak etanol daun bidara (Ziziphus mauritiana L.) sebagai anti jerawat terhadap bakteri Propionibacterium acnes dan Staphylococcus aureus, 4(3), 249–264.
Artdita, C. A., Aziz, F., Hidayah, N., Fauzi, A., Wulandari, T. S., & Hamid, R. L. (2021). Identifikasi molekuler bakteri Staphylococcus sp. dan Staphylococcus aureus penyebab mastitis subklinis pada ternak kambing perah. Jurnal Sain Veteriner, 39(2), 151. https://doi.org/10.22146/jsv.60557
Avila-Nava, A., Acevedo-Carabantes, J. A., Alamilla-Martinez, I., Tobón-Cornejo, S., Torre-Villalvazo, I., Tovar, A. R., Torres, N., & Noriega, L. G. (2023). Chaya (Cnidoscolus aconitifolius (Mill.) I.M. Johnst) leaf extracts regulate mitochondrial bioenergetics and fatty acid oxidation in C2C12 myotubes and primary hepatocytes. Journal of Ethnopharmacology, 312, 116522. https://doi.org/10.1016/j.jep.2023.116522
Badan Standardisasi Nasional. (2021). SNI 3532:2021 sabun mandi. Jakarta, Indonesia: Badan Standardisasi Nasional.
Butzge, J. C., Pivotto, C., Mezzomo, L., Ferrão, S. K., Picanço, J. M. A., Mezzari, A., Calil, L. N., Limberger, R. P., & Apel, M. A. (2023). Antifungal properties of essential oils derived from the genus Cymbopogon: A systematic review. Chemistry & Biodiversity, 20(10), e202300663. https://doi.org/10.1002/cbdv.202300663
Das, S., Agarwal, S., Samanta, S., Kumari, M., & Das, R. (2024). Formulation and evaluation of herbal soap. Journal of Pharmacognosy and Phytochemistry, 13(4), 14–19. https://doi.org/10.22271/phyto.2024.v13.i4a.14990
Dimpudus, S. A., Yamlean, P. V. Y., & Yudistira, A. (2023). Formulasi sediaan sabun cair antiseptik ekstrak etanol bunga pacar air (Impatiens balsamina L.) dan uji efektivitasnya terhadap bakteri Staphylococcus aureus secara in vitro. Jurnal Farmasi.
GBD 2021 Antimicrobial Resistance Collaborators. (2024). Global burden of bacterial antimicrobial resistance 1990–2021: A systematic analysis with forecasts to 2050. The Lancet, 404(10459), 1199–1226. https://doi.org/10.1016/S0140-6736(24)01867-1
Gehrke, A.-K. E., Giai, C., & Gómez, M. I. (2023). Staphylococcus aureus adaptation to the skin in health and persistent/recurrent infections. Antibiotics, 12(10), 1520. https://doi.org/10.3390/antibiotics12101520
Giacobbe, D. R., Dettori, S., Corcione, S., Vena, A., Sepulcri, C., Maraolo, A. E., De Rosa, F. G., & Bassetti, M. (2022). Emerging treatment options for acute bacterial skin and skin structure infections and bloodstream infections caused by Staphylococcus aureus: A comprehensive review of the evidence. Infection and Drug Resistance, 15, 2137–2157. https://doi.org/10.2147/IDR.S318322
Howden, B. P., Giulieri, S. G., Lung, T. W. F., Baines, S. L., Sharkey, L. K., Lee, J. Y. H., Hachani, A., & Monk, I. R. (2023). Staphylococcus aureus host interactions and adaptation. Nature Reviews Microbiology, 21(6), 380–395. https://doi.org/10.1038/s41579-023-00852-y
Huitema, L., Phillips, T., Alexeev, V., Tomic-Canic, M., Pastar, I., & Igoucheva, O. (2021). Intracellular escape strategies of Staphylococcus aureus in persistent cutaneous infections. Experimental Dermatology, 30, 1428–1439. https://doi.org/10.1111/exd.14235
Kartika, D. I. N., Rahmadani, A., Lestari, S., Putri, N. A., Fatah, M., & Nurjamah, S. I. (2023). Formulation and evaluation of liquid soap with essential oil from olive (Olea europaea var. europaea). Indonesian Journal of Health Science, 3(2a), 229–236.
Kurniawati, A., Eltivitasari, A., & Trilestari, T. (2025). Formulasi sediaan sabun padat ekstrak etanol serai wangi (Cymbopogon nardus L.). Sinteza, 5(1), 1–10. https://doi.org/10.29408/sinteza.v5i1.26921
Linz, M. S., Mattappallil, A., Finkel, D., & Parker, D. (2023). Clinical impact of Staphylococcus aureus skin and soft tissue infections. Antibiotics, 12(3), 557. https://doi.org/10.3390/antibiotics12030557
Mishra, P., Pandey, C. M., Singh, U., & Gupta, A. (2019). Descriptive statistics and normality tests for statistical data. Annals of Cardiac Anaesthesia, 22(1), 67–72. https://doi.org/10.4103/aca.ACA_157_18
Mopangga, E., Yamlean, P. V. Y., & Abdullah, S. S. (2021). Formulation of solid bath soap with ethanol extract of gedi leaves (Abelmoschus manihot L.) to Staphylococcus epidermidis bacteria. Pharmacon, 10(3), 1017–1025.
Ningrum, D. K., Wiyono, A. E., & Amilia, W. (2021). Evaluasi mutu sabun padat dengan penambahan variasi ekstrak etanol tembakau (Nicotiana tabacum L.). EnviroScienteae, 17(2), 48. https://doi.org/10.20527/es.v17i2.11494
Novita Sari, S., Prastiwi, R., & Hayati, H. (2022). Studi farmakognosi, fitokimia dan aktivitas farmakologi tanaman pepaya Jepang (Cnidoscolus aconitifolius (Mill.) I.M. Johnston). Farmasains: Jurnal Ilmiah Ilmu Kefarmasian, 9(1), 19–28. https://doi.org/10.22236/farmasains.v9i1.5403
Padilla-Camberos, E., Torres-Gonzalez, O. R., Sanchez-Hernandez, I. M., Diaz-Martinez, N. E., Hernandez-Perez, O. R., & Flores-Fernandez, J. M. (2021). Anti-inflammatory activity of Cnidoscolus aconitifolius (Mill.) ethyl acetate extract on croton oil-induced mouse ear edema. Applied Sciences, 11(20), 9697. https://doi.org/10.3390/app11209697
Panghal, A., Shaji, A. O., Nain, K., Garg, M. K., & Chhikara, N. (2021). Cnidoscolus aconitifolius: Nutritional, phytochemical composition and health benefits—A review. Bioactive Compounds in Health and Disease, 4(11), 260–286. https://doi.org/10.31989/BCHD.V4I11.865
Piasecki, B., Biernasiuk, A., Skiba, A., Skalicka-Woźniak, K., & Ludwiczuk, A. (2021). Composition, anti-MRSA activity and toxicity of essential oils from Cymbopogon species. Molecules, 26(24), 7542. https://doi.org/10.3390/molecules26247542
Rosmania, F. (2020). Perhitungan jumlah bakteri di laboratorium mikrobiologi menggunakan pengembangan metode spektrofotometri. 22(2), 76–86.
Saputra, N. A., Wibisono, H. S., Darmawan, S., & Pari, G. (2020). Chemical composition of Cymbopogon nardus essential oil and its broad spectrum benefit. IOP Conference Series: Earth and Environmental Science, 415(1). https://doi.org/10.1088/1755-1315/415/1/012017
Suarantika, F., Patricia, V. M., & Rahma, H. (2023). Karakterisasi dan identifikasi senyawa minyak atsiri pada sereh wangi (Cymbopogon nardus (L.) Rendle) dengan kromatografi gas-spektrometri massa. Jurnal Mandala Pharmacon Indonesia, 9(2), 514–523.
Susilowati, M., & Syukur, C. (2022). Karakterisasi beberapa aksesi serai wangi (Cymbopogon nardus L.) asal Cianjur. Vegetalika, 11(4), 305. https://doi.org/10.22146/veg.77033
Trindade, L. A., Cordeiro, L. V., Silva, D. F., Figueiredo, P. T. R., Pontes, M. L. C., Lima, E. O., & Carvalho, A. A. T. (2022). The antifungal and antibiofilm activity of Cymbopogon nardus essential oil and citronellal on clinical strains of Candida albicans. Brazilian Journal of Microbiology, 53(3), 1231–1240. https://doi.org/10.1007/s42770-022-00740-2
Vargas-Madriz, Á. F., Luzardo-Ocampo, I., Moreno-Celis, U., Roldán-Padrón, O., Chávez-Servín, J. L., Vergara-Castañeda, H. A., Martínez-Pacheco, M., Mejía, C., García-Gasca, T., & Kuri-García, A. (2023). Comparison of phytochemical composition and untargeted metabolomic analysis of an extract from Cnidoscolus aconitifolius (Mill.) I. I. Johnst and Porophyllum ruderale (Jacq.) Cass. and biological cytotoxic and antiproliferative activity in vitro. Plants, 12(10), 1987. https://doi.org/10.3390/plants12101987
Yanto, R. B., Satriawan, N. E., & Suryani, A. (2021). Identifikasi dan uji resistensi Staphylococcus aureus terhadap antibiotik (chloramphenicol dan cefotaxime sodium). Jurnal Kimia Riset, 6(2), 154.
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Hak Cipta (c) 2026 Chofifatun Mubarokah, Anna Fitriawati, Bagas Ardiyantoro (Author)
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